Levent Keskintepe, PhD

El Dr. Keskintepe aporta más de dos décadas de experiencia en dirección de laboratorios de FIV e investigación. Su trabajo combina rigor científico, sistemas de laboratorio cuidadosos y coordinación estrecha con el equipo clínico.

Dirección del laboratorio

Cuenta con las acreditaciones estadounidenses High-complexity Clinical Laboratory Director y Clinical Consultant del American Board of Bioanalysis, y con amplia experiencia en reproducción asistida y criobiología.

Áreas de trabajo

• Embriología clínica
• Operaciones del laboratorio de FIV
• Cultivo embrionario y criopreservación
• Sistemas de calidad del laboratorio

Formación y experiencia

Obtuvo su doctorado en Fisiología Reproductiva en la University of Georgia (1990–1994), donde realizó formación posdoctoral (1994–1996). Su currículum recoge la dirección de laboratorios en SIRM, LAIVF y RPMG, además de puestos académicos e investigación en desarrollo embrionario, criopreservación y genética reproductiva.

Publicaciones y contribuciones científicas

La bibliografía incluye los 34 registros de PubMed para Keskintepe L comprobados el 5 de octubre de 2026, seguidos de otros trabajos recogidos en su currículum. Se conservan los títulos originales para facilitar su identificación y consulta; deben leerse en su contexto histórico y científico.

Consultar currículum y bibliografía completa (PDF en inglés): incluye las 45 entradas de manuscritos, 68 resúmenes y actas y 22 presentaciones invitadas del currículum aportado, con notas sobre las fuentes.

Publicaciones indexadas en PubMed y capítulos de metodología (títulos originales)

1. Agca Y, Keskintepe L. Cryopreservation in human reproductive medicine. Syst Biol Reprod Med. 2025;71(1):598-617. PubMed 41340379
2. Keskintepe L, Eroglu A. Preservation of Mammalian Sperm by Freeze-Drying. Methods Mol Biol. 2021;2180:721-730. PubMed 32797445
3. Tortoriello DV, Dayal M, Beyhan Z, Yakut T, Keskintepe L. Reanalysis of human blastocysts with different molecular genetic screening platforms reveals significant discordance in ploidy status. J Assist Reprod Genet. 2016;33(11):1467-1471. PubMed 27423662
4. Keskintepe L, Eroglu A. Freeze-drying of mammalian sperm. Methods Mol Biol. 2015;1257:489-97. PubMed 25428025
5. Kotze D, Kruger TF, Lombard C, Padayachee T, Keskintepe L, Sher G. The effect of the biochemical marker soluble human leukocyte antigen G on pregnancy outcome in assisted reproductive technology--a multicenter study. Fertil Steril. 2013;100(5):1303-9. PubMed 23993930
6. Kotze D, Keskintepe L, Sher G, Kruger T, Lombard C. A linear karyotypic association between PB-I, PB-II and blastomere using sequentially performed comparative genome hybridization with no association established between karyotype, morphologic, biochemical (sHLA-G expression) characteristics, blastocyst formation and subsequent pregnancy outcome. Gynecol Obstet Invest. 2012;74(4):304-12. PubMed 22890181
7. Yakut T, Karkucak M, Sher G, Keskintepe L. Comparison of aneuploidy frequencies between in vitro matured and unstimulated cycles oocytes by metaphase comparative genomic hybridization (mCGH). Mol Biol Rep. 2012;39(5):6187-91. PubMed 22207182
8. Kotze DJ, Hansen P, Keskintepe L, Snowden E, Sher G, Kruger T. Embryo selection criteria based on morphology VERSUS the expression of a biochemical marker (sHLA-G) and a graduated embryo score: prediction of pregnancy outcome. J Assist Reprod Genet. 2010;27(6):309-16. PubMed 20358276
9. Keskintepe L, Sher G, Machnicka A, Tortoriello D, Bayrak A, Fisch J, Agca Y. Vitrification of human embryos subjected to blastomere biopsy for pre-implantation genetic screening produces higher survival and pregnancy rates than slow freezing. J Assist Reprod Genet. 2009;26(11-12):629-35. PubMed 19967555
10. Sher G, Keskintepe L, Keskintepe M, Maassarani G, Tortoriello D, Brody S. Genetic analysis of human embryos by metaphase comparative genomic hybridization (mCGH) improves efficiency of IVF by increasing embryo implantation rate and reducing multiple pregnancies and spontaneous miscarriages. Fertil Steril. 2009;92(6):1886-94. PubMed 19135663
11. Keskintepe L, Agca Y, Sher G, Keskintepe M, Maassarani G. High survival rate of metaphase II human oocytes after first polar body biopsy and vitrification: determining the effect of previtrification conditions. Fertil Steril. 2009;92(5):1706-15. PubMed 18937944
12. Sher G, Keskintepe L, Mukaida T, Keskintepe M, Ginsburg M, Agca Y, Maassarani G, Bayrak A. Selective vitrification of euploid oocytes markedly improves survival, fertilization and pregnancy-generating potential. Reprod Biomed Online. 2008;17(4):524-9. PubMed 18854107
13. Keskintepe L, Sher G, Keskintepe M. Reproductive oocyte/embryo genetic analysis: comparison between fluorescence in-situ hybridization and comparative genomic hybridization. Reprod Biomed Online. 2007;15(3):303-9. PubMed 17854528
14. Keskintepe L, Norris K, Pacholczyk G, Dederscheck SM, Eroglu A. Derivation and comparison of C57BL/6 embryonic stem cells to a widely used 129 embryonic stem cell line. Transgenic Res. 2007;16(6):751-8. PubMed 17701442
15. Fisch JD, Keskintepe L, Sher G. Gonadotropin-releasing hormone agonist/antagonist conversion with estrogen priming in low responders with prior in vitro fertilization failure. Fertil Steril. 2008;89(2):342-7. PubMed 17562336
16. Sher G, Keskintepe L, Keskintepe M, Ginsburg M, Maassarani G, Yakut T, Baltaci V, Kotze D, Unsal E. Oocyte karyotyping by comparative genomic hybridization [correction of hybrydization] provides a highly reliable method for selecting "competent" embryos, markedly improving in vitro fertilization outcome: a multiphase study. Fertil Steril. 2007;87(5):1033-40. PubMed 17258713
17. Fisch JD, Keskintepe L, Ginsburg M, Adamowicz M, Sher G. Graduated Embryo Score and soluble human leukocyte antigen-G expression improve assisted reproductive technology outcomes and suggest a basis for elective single-embryo transfer. Fertil Steril. 2007;87(4):757-63. PubMed 17224145
18. Sher G, Keskintepe L, Ginsburg M. sHLA-G expression: is it really worth measuring? Reprod Biomed Online. 2007;14(1):9-10. PubMed 17207322
19. Sher G, Keskintepe L, Fisch JD, Acacio BA, Ahlering P, Batzofin J, Ginsburg M. Soluble human leukocyte antigen G expression in phase I culture media at 46 hours after fertilization predicts pregnancy and implantation from day 3 embryo transfer. Fertil Steril. 2005;83(5):1410-3. PubMed 15866577
20. Sher G, Keskintepe L, Batzofin J, Fisch J, Acacio B, Ahlering P, Ginsburg M. Influence of early ICSI-derived embryo sHLA-G expression on pregnancy and implantation rates: a prospective study. Hum Reprod. 2005;20(5):1359-63. PubMed 15746200
21. Sher G, Keskintepe L, Nouriani M, Roussev R, Batzofin J. Expression of sHLA-G in supernatants of individually cultured 46-h embryos: a potentially valuable indicator of 'embryo competency' and IVF outcome. Reprod Biomed Online. 2004;9(1):74-8. PubMed 15257824
22. Fisch JD, Sher G, Adamowicz M, Keskintepe L. The graduated embryo score predicts the outcome of assisted reproductive technologies better than a single day 3 evaluation and achieves results associated with blastocyst transfer from day 3 embryo transfer. Fertil Steril. 2003;80(6):1352-8. PubMed 14667868
23. Keskintepe L, Pacholczyk G, Machnicka A, Norris K, Curuk MA, Khan I, Brackett BG. Bovine blastocyst development from oocytes injected with freeze-dried spermatozoa. Biol Reprod. 2002;67(2):409-15. PubMed 12135874
24. Keskintepe L, Agca Y, Pacholczyk GA, Machnicka A, Critser JK. Use of cryopreserved pronuclear embryos for the production of transgenic mice. Biol Reprod. 2001;65(2):407-11. PubMed 11466207
25. Wrenzycki C, Herrmann D, Keskintepe L, Martins A, Sirisathien S, Brackett B, Niemann H. Effects of culture system and protein supplementation on mRNA expression in pre-implantation bovine embryos. Hum Reprod. 2001;16(5):893-901. PubMed 11331635
26. Keskintepe L, Brackett BG. Cryopreservation of bovine blastocysts obtained by intracytoplasmic sperm injection. Theriogenology. 2000;53(5):1041-52. PubMed 10798482
27. Keskintepe L, Simplicio AA, Brackett BG. Caprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders. Theriogenology. 1998;49(7):1265-74. PubMed 10732064
28. Servy EJ, Kaufmann RA, Liu Z, Menezo Y, Keskintepe L. Human pregnancies after transfer of fresh (four- to eight-cell) versus frozen-thawed blastocysts resulting from intracytoplasmic sperm injection. J Assist Reprod Genet. 1998;15(7):422-6. PubMed 9717117
29. Keskintepe L, Morton PC, Smith SE, Tucker MJ, Simplicio AA, Brackett BG. Caprine blastocyst formation following intracytoplasmic sperm injection and defined culture. Zygote. 1997;5(3):261-5. PubMed 9460911
30. Keskintepe L, Brackett BG. In vitro developmental competence of in vitro-matured bovine oocytes fertilized and cultured in completely defined media. Biol Reprod. 1996;55(2):333-9. PubMed 8828837
31. Luvoni GC, Keskintepe L, Brackett BG. Improvement in bovine embryo production in vitro by glutathione-containing culture media. Mol Reprod Dev. 1996;43(4):437-43. PubMed 9052934
32. Keskintepe L, Burnley CA, Brackett BG. Production of viable bovine blastocysts in defined in vitro conditions. Biol Reprod. 1995;52(6):1410-7. PubMed 7632849
33. Keskintepe L, Darwish GM, Younis AI, Brackett BG. In vitro development of morulae from immature caprine oocytes. Zygote. 1994;2(2):97-102. PubMed 7874460
34. Keskintepe L, Darwish GM, Kenimer AT, Brackett BG. Term development of caprine embryos derived from immature oocytes in vitro. Theriogenology. 1994;42(3):527-35. PubMed 16727559

Additional works reported in the source CV

These works are supplied-CV references, including proceedings and a book. They were not independently confirmed in the PubMed search. The source CV combines published and submitted manuscripts; this section does not imply that every entry is an indexed journal article.

• Diker K.S., Erdeger J., Keskintepe L., and Arda M. (1987). Isolation of campylobacter coli from a cow with mastitis. Fac. Vet. Med. Univ. Ankara 34:500-502.
• Keskintepe L., Kuplulu S.A., and Alpar R. (1988). Studies on reproductive performance of pure breed Arabic mares in Cifteler Anadolu Tarim Isletmesi. Fac. Vet. Med. Univ. Ankara 35:488-496.
• Keskintepe L., Vural R., and Izgur H. 1990). Impact of using DMSO as dissolving agent for different antibiotics in cows with mastitis. Fac. Vet. Med. Univ. Ankara 39:348- 356.
• Brackett B.G. and Keskintepe L. (1994). Aspects of bovine oocyte maturation and embryo production in vitro. 7th International Meeting of Animal Reproduction, Spanish Association on Animal Reproduction, Murcia, Spain, pp 27-30.
• Keskintepe L., Luvoni G.C., Rzucidlo S.J. and Brackett B.G. (1996). Procedural improvements for in vitro production of viable uterine stage caprine embryos. Small Ruminant research 20:247-254.
• Keskintepe L., Menenzo Y., Kaufmann R.A., Gilchrist F.H., and Servy E.J. (1997). Live offspring after cryopreservation of cocultured human blastocysts fertilized by intracytoplasmic sperm injection (ICSI). Proc. 10th World Congress on In vitro fertilization and Assisted Reproduction, Vancouver, Canada 24-28 May 1997. (Ed. Gomel, V., Leung, P.C.K.), published by Monduzzi Editore, pp. 65-69.
• Bagis H., Keskintepe L., Odaman H, Sarikaya H. Techniques to generate Transgenic Animal. TUBITAK Press 2000.
• Bagıs H., Keskintepe L. (2001). Application of green fluorescent protein as a marker for selection of transgenic mouse embryos before implantation. Turkish Journal of Biology 35: 204-216.
• Bagis H., Keskintepe L., Odaman H. Sagirkaya H. (2001). Effect of CZB medium on the two-cell block of preimplantation mouse embryos. Turkish Journal of Veterinary and Animal Science, 25:725-729.
• Bagis H., Keskintepe L., Odaman H. Sagirkaya H., Cetin S. (2001). Influences of cumulus cells during in vitro fertilization of mouse oocytes in different mouse strain. Turkish Journal of Veterinary and Animal Science, 25:777-782.
• Bagis H., Keskintepe L., Odaman H., Sagirkaya H., Sekmen S. (2002). Comparing the birth rates of microinjected hybrid mouse zygotes transferred into outbreed and hybrid foster mothers. Turkish Journal of Veterinary and Animal Science, 26:210-216.
• Sirisathien S, Keskintepe L, Brackett B. (2003). Bull Sperm Uptake of Exogenous DNA and Efforts to Obtain Transgenic Embryos. Transgenics 4 (1): 23-28.
• Keskintepe. L (2004). Assessing sHLA-G secretion: a useful marker for embryo selection on day 3. Fertility World 2:12.
• Keskintepe L and Beyhan Z (2010). Intracytoplasmic Sperm Injection (ICSI) and the factors affecting the immediate outcome: fertilization and preimplantation development. J. Reprod. Stem Cell Biotechnol. 1(2) 193-211, 2010.

Abstracts, proceedings and invited presentations

The full PDF preserves all 68 scientific presentations, abstracts and proceedings, plus 22 invited lectures, separately from the manuscript bibliography. It also flags a possible duplicate manuscript entry and a source date requiring clarification.

The professional-history source is the CV supplied in February 2025. The public copy omits personal contact details and separately dates the updated PubMed bibliography.